Phage display library construction protocol

 

 

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Michelle A. Clark 1. show more details. Series: Methods In Molecular Biology > Book: Antibody Phage Display. Protocol | DOI: 10.1385/1-59259-240-6:039.cDNA libraries are constructed in a state-of-the-art phagemid vector (pHD9). Use the following protocol to titer the phage display library before you Phage Display Library Construction · Hard randomization: complete diversity is introduced by using degenerate codons encoding all twenty natural amino acids.

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